RESEARCH ASSISTANT/PhD POSITION

Posted on June 23,

Institute of Molecular Biology and Biotechnology, Faculty of Biology & Center for Advanced Technology, Adam Mickiewicz University, Poznań, Poland

Link:

http://ibmib.amu.edu.pl/wp-content/uploads/2022/06/ERC-PhD-ad.pdf
https://www.euraxess.pl/jobs/796210

We are seeking a highly motivated research assistant wanting to pursue a PhD to join the GENOMIC REGULATION LAB led by DR HAB. KINGA KAMIENIARZ-GDULA at Adam Mickiewicz University, Poznań, Poland. You will be offered the opportunity to participate in cutting edge research and lead your own project aiming at understanding how alternative gene ends are selected in human cells.

The full-time position is funded for four years by the prestigious ERC STARTING GRANT “AlternativeEnds”. There will be a probation period of 6-12 months. The yearly gross (brutto brutto) salary will be between 80.000 PLN (minimum pay during probation) and 100.000 PLN (projected pay for the remainder of the project). According to Horizon Europe regulations, the position is based on full-time research employment. The successful candidate is expected to obtain a PhD degree based on the research done within the project, via the extramural doctorate pathway. 

Expected start date: 1st October 2022.
Application deadline: 10th July 2022.

Project:

The human genome contains only ~20.000 genes, however, most of them encode multiple transcripts resulting from alternative promoter usage, splicing, and 3’ end selection. Gene 3’ ends can be defined by the positions of RNA 3’ cleavage, or the location where RNA polymerase II terminates transcription. Alternative 3’ ends determine the properties of the encoded protein: typically its abundance, but sometimes also domain structure – as for immunoglobulin M heavy chain which is membrane-bound or secreted depending on the 3’ cleavage site. Widespread changes in 3’ end usage are characteristic of many processes e.g. differentiation and cancer. We do not understand what drives this selectivity.

In order to find out the determinants of gene end selection we will study the crosstalk of RNA cleavage and transcription termination. We recently pioneered the measurement of 3’ cleavage positions together with locations of transcription termination by a novel transcriptomic method. You will apply this method as well as develop new NGS techniques to investigate changes in cleavage and termination.

Ultimately, understanding the complex crosstalk between RNA cleavage and transcription termination in alternative 3’ end selection will enable the manipulation of this process e.g. to alleviate human disease.

Tasks:

Leading own research project: planning and conducting molecular biology and cell culture experiments as well as computational analysis of NGS data. Presenting results in group meetings, internal seminars and conferences, manuscript preparation.

Essential qualifications:

- MSc in Molecular Biology/Biotechnology or a related science degree (obtained by 30 Sept 2022)
- experience in standard Molecular Biology/Biochemistry techniques
- high motivation and enthusiasm for research
- fluency in English

Desirable qualifications (training will be provided whenever necessary):

- experience with NGS techniques (particularly RNA-seq and ChIP-seq)
- experience with cell culture and cell differentiation
- bioinformatic skills
- co-authorship in publications from a relevant subject

Interested candidates should send: a cover letter, CV and the contacts of three referees as a single pdf file to: [email protected]. The cover letter should explain the candidate’s motivation to join the team.

Informal inquiries and questions welcome.

Please include in the CV and cover letter the following statement: In accordance with Article 6(1)(a) of the General Data Protection Regulation of 27 April 2016 (Journal of Laws of the EU L 119/1 of 4 May 2016) I agree to the processing of personal data other than those indicated in Article 221 of the Labour Code (name(s) and surname; parents' names; date of birth; place of residence; address for correspondence; education; previous employment), included in my job offer for the purpose of current recruitment.

Lab webpage:
https://STOPlab.org