The laboratory of Functional Genomics and Noncoding RNAs at the Montreal Clinical Research Institute (IRCM) is looking for a highly motivated and resourceful individual to fill the position of Research Assistant. The candidate will be part of a team studying noncoding regions of the genome to uncover new functional elements and understand how they contribute to human diseases. We are particularly interested in long noncoding RNAs (lncRNAs) and cis regulatory modules and use genome editing, genomics, molecular and bioinformatics approaches to characterize their function. The candidate will play a central role in the laboratory and help maintain a nurturing and stimulating environment.
Summary of Responsibilities
Under the direction of Dr. Martin Sauvageau, the candidate will be responsible to manage and help coordinate the day-to-day lab operations, order reagents, perform experiments and assist in training new lab members. The candidate will be expected to implement current technologies in ongoing research projects, adapt the technologies to new applications and ensure the quality of the data being produced. Additional tasks will be to carry out cloning, primary cell cultures, CRISPR genome editing, harvest tissues from animal models, help prepare samples and perform functional and biochemical assays, help identify and implement new approaches for solving relevant biological problems.
- Carry out molecular cloning, purification and expression of RNA and protein constructs
- Work closely with Principal Investigator and Research Associate to help develop and carry out biochemical and functional assays to study RNA and protein function
- Contribute to the development, implementation and optimization of lab protocols and organizational framework to create efficient lab workflows
- Perform mouse genotyping and manage mouse colonies together with the mouse technician
- Help with day-today lab operations (reagent preparation, instrument maintenance, ordering)
- Maintain a detailed laboratory notebook
- Present and discuss experimental findings and plans in meetings with the Principal Investigator and research group
- Help ensure and enforce lab safety standards
- Perform miscellaneous job-related duties as assigned
- MSc degree in the life sciences or BSc degree with at least 2-3 years of job-related experience
- Strong molecular biology background
- Experience in a molecular biology and/or biochemistry research lab
- Experience in molecular cloning, RNA & Protein purification, cell culture
- Knowledge/experience with standard biochemical and molecular biology assays. Experience with RNA-protein interactions is a plus
- Knowledge/experience with quantitative analysis techniques for RNA and protein. Experience with next-generation sequencing and/or bioinformatic is a plus
- Experience with mouse manipulation and experimental work is a plus
- Excellent organizational and time management skills
- Ability to multitask, set priorities, and thrive in a fast-paced environment
- Excellent problem-solving skills
- Effective communication skills
- Critical thinking skills who excels at completing projects in an independent manner
- Enthusiasm and is eager to acquire new knowledge and skills on a regular basis
- Fluency in English (writing and speaking)
How to apply
Applicants should submit a one-page statement letter explaining why you’re interested in our work, a detailed CV and the names and contact information for 3 references to:
Dr. Martin Sauvageau
Director of the Functional Genomics and Noncoding RNA Laboratory
The applications will be examined as they are received, until the position is filled. Only those applicants selected for an interview will be contacted.
The Montreal Clinical Research Institute (IRCM) is an independent non-profit research institution with a dynamic and multi-disciplinary team of scientists working on a wide range of basic and clinical research subjects. Located in downtown Montreal, it is affiliated with the Université de Montréal and McGill University. The institute represents an excellent working environment, both in terms of the diversity of subjects and systems studied. The modern infrastructure of the IRCM houses state-of-the-art equipment and core facilities for a variety of applications in functional genomics, proteomics, imaging, flow cytometry, molecular biology and bioinformatics.